Part:BBa_K3071014:Design
gumB CBS I & II-regulated pspA promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 72
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA spacing between CBS I, CBS II and pspA promotor are considered as it controls the availability of protein interaction. CBS I and CBS II on the UAS region are referred to as the original spacing in the Xcc bacteria genome. The spacing between the UAS and the pspA promotor is also referred to as the original genome of E. coli.
References
Chen, C. H., Lin, N. T., Hsiao, Y. M., Yang, C. Y., & Tseng, Y. H. (2010). Two non-consensus Clp binding sites are involved in upregulation of the gum operon involved in xanthan polysaccharide synthesis in Xanthomonas campestris pv. campestris. Research in microbiology, 161(7), 583-589.
Dworkin, J., Jovanovic, G., & Model, P. (1997). Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. Journal of molecular biology, 273(2), 377-388.
Rappas, M., Schumacher, J., Beuron, F., Niwa, H., Bordes, P., Wigneshweraraj, S., ... & Zhang, X. (2005). Structural insights into the activity of enhancer-binding proteins. Science, 307(5717), 1972-1975.
Wigneshweraraj, S., Bose, D., Burrows, P. C., Joly, N., Schumacher, J., Rappas, M., ... & Buck, M. (2008). Modus operandi of the bacterial RNA polymerase containing the σ54 promoter‐specificity factor. Molecular microbiology, 68(3), 538-546.
Zhou, Y., Asahara, H., Schneider, N., Dranchak, P., Inglese, J., & Chong, S. (2014). Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays. Journal of the American Chemical Society, 136(40), 14031-14038.